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KMID : 0616620000060020313
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Journal of Soonchunhyang Medical College
2000 Volume.6 No. 2 p.313 ~ p.319
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Inhibition Effect of Angiogenesis with Human Endostatin
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Abstract
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Background : Angiogenesis is a critical determinant of tumor growth and the development of metastasis. Endostatin have been used in a variety of in vitro and in vivo animal models as effective inhibitors of angiogenesis. However, human endostatin have not tested against an intact human tissue target in vitro to determine its ability of antiangiogenic response. Purpose : We performed our study to determine that human endostatin would inhibit the development of an angiogenic response (initiation) and the subsequent growth (angiogenic index) of the human vessels in a dose-dependent manner with a human placental vein angiogenesis model(HPVAM). Methods : We used full thickness human placental vein discs cultured in three-dimensional fibrin-thrombin clots with an overlay of liquid media. Human endostatin were evaluated in concentrations ranging from 1x10-9M/L to 1x10-4M/L. A positive control containing 20% fetal bovine serum and a negative control using heparin and hydrocortisone 21-phosphate were also tested. Reuslts : Human endostatin significantly inhibited the initiation rate of an angiogenic response at concentration of 1x10-4M/L(p<0.001) and the subsequent development of the angiogenic response (angiogenic index) at concentrations of 1x10-5M/L and 1x10-4M/L(p<0.001, p<0.001 respectively). Conclusions : We concluded that the very high concentration of human endostatin can inhibit the angiogenic response in the human blood vessels in vitro. These results suggest that human endostatin has a powerful antiangiogenic effect against an intact human tissue target.
Key word : Endostatin, Angiogenesis, Antiangiogenesis, Human placental vein angiogenesis model
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KEYWORD
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